Demethylation reactions were conducted with 2 μl of the immobilized anti-FLAG bead suspension with peptide (10 μM) and cofactors/substrates (conditions given in Supplementary Table 3).

Reversing this modification requires DNA demethylation, a series of enzymatic reactions which starts with the creation of 5-hydroxymethylcytosine (5hmC).

Post-translational gene expression is regulated by ALKBH1, an enzyme that demethylates transfer RNA.

SALL4 up-regulation after treatment with hypomethylating agents was observed in 10 of 25 patients (40%) in cohort 1 and in 13 of 43 patients (30%) in cohort 2 and was associated with a worse ...

Methylation-specific polymerase chain reaction, bisulfite genomic sequencing, and immunohistochemistry were used to assess demethylation and gene re-expression. Results. Substantial degrees of ...

Looking at prior research data, the team found that as tomatoes became more domesticated over the past few centuries, ...

Mechanism behind demethylation pinpointed in APC gene mutants. ScienceDaily . Retrieved May 24, 2025 from www.sciencedaily.com / releases / 2010 / 09 / 100917183031.htm ...

Lochmann TL, Powell KM, Ham J, et al. Targeted inhibition of histone H3K27 demethylation is effective in high-risk neuroblastoma. Sci Transl Med 2018;10(441):eaao4680-eaao4680.

Methylation-specific polymerase chain reaction, bisulfite genomic sequencing, and immunohistochemistry were used to assess demethylation and gene re-expression. Results. Substantial degrees of ...