investigators will also stab several times through the agar using an inoculating loop. This stab allows for the detection of streptolysin O, a specific hemolysin produced by Streptococcus pyogenes.
An inoculating loop can be used to transfer bacteria. It is sterilised by heating it to red hot in a Bunsen flame, before and after use. To inoculate the agar, lift the lid of the Petri dish and tilt.
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