Chimeric antigen receptor (CAR) T cell therapy is a novel cellular therapeutic ... in partnership with ELRIG (UK) Ltd. Multiplexed CAR expression and apoptosis assay can detect the percentage ...

and these target cells displayed rounding/detachment and cytoplasmic shrinkage, characteristic of progressing through apoptosis. Repeating the assay while using a fixed E:T of 4:1 clearly ...

Apoptosis reagents must be diluted to ensure compatibility of apoptotic marker and target cell label, and treatments (such as cytokines, T cell stimuli, and antibodies) at 4 times final assay ...

Unexpectedly, we found that some of the assays widely used to detect apoptosis do not distinguish ... understand the mechanism of cell death. The Jurkat T-lymphoma and Ramos and Raji B-lymphoma ...

In this work, the researchers exploited apoptosis-associated changes in morphology to develop a CC3-based assay that is highly specific for detecting apoptotic cells. By employing an image ...

Studies carried out till date to elucidate the pathways involved in HIV-1-induced T-cell depletion has revealed that apoptosis underlie ... intracellular p24 antigen assay and HIV-1 gag-specific ...

The new method is called RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay. Viable cells ensure their physical and functional integrity through a highly ordered membrane structure consisting ...

Modulating lncRNA NKILA in tumor-reactive T cells to enhance their trafficking to breast cancer by inhibition of FasL-induced T-cell apoptosis. This is an ASCO Meeting Abstract from the 2017 ASCO-SITC ...

Cellular therapies could play a role in cancer treatment and regenerative medicine if it were possible to quickly eliminate the infused cells in case of adverse events. We devised an inducible T ...

But as most labs don't have EM technicians on staff ... Other than caspase activation, none of these tests indicates apoptosis so much as cell death in general. Reed's recommendations: run multiple ...

Excitingly, these finding support that “this assay can be used in ongoing translational research studies to investigate CAR-T cell and TCR-T cell trafficking,” commented the authors. “Insight into ...

These findings suggest a direct effect of COYA 303 supporting Treg survival through the inhibition of Treg apoptosis ... advantages of regulatory T cells (“Tregs”) to target systemic ...