This Nikon Ti-U is an inverted widefield epifluorescence microscope with both fluorescence and color imaging (RGB, histology). For fluorescence, this microscope uses an Excelitas Excite 100-watt metal ...

This diffraction causes the light to form a diffraction pattern, known as the Airy disk, at the focal plane of the microscope. The Airy disk consists of a central bright spot surrounded by concentric ...