Capstan Therapeutics scientists demonstrate that lipid nanoparticles can engineer CAR T cells within the body without ...

Image Credit: ACROBiosystems NK cells were co-cultured with cells derived from K562. This was done at E:T ratios of 0.625:1, 1.25:1, 2.5:1, 5:1, and 10:1. Next, 7-AAD/CFSE staining was used to ...

NK cells were washed with PBS before incubation with MDMs and Dil-Ox-LDL. After incubation for 4 hours, cells were subsequently washed twice with PBS, and the intensity of DiI staining was measured ...

Immunometabolic reprogramming due to adenosine produced by CD73 (encoded by the 5’-ectonucleotidase gene NT5E) is a recognized immunosuppressive mechanism contributing to immune evasion in solid ...

NK cell differentiation was quantified by flow cytometry. Isolated cells were stained with Live/Dead Fixable Dead Cell Stain (ThermoFisher Scientific) and marker staining performed with panels of ...

In hematologic oncology, advancements in chimeric antigen receptors (CARs) for T-cell therapy have led to new investigations and an emerging role for CAR-natural killer (NK) cell therapy. Here we ...

C. Gating strategy to define YTS‐2DL1 incorporated NKp46 antibody‐coated NPs population, using anti‐KIR2DL1 to define NK cells. Cells that were positive for both rhodamine‐labeled NPs and KIR2DL1 ...

NK cells kill target cells without the need for prior sensitization ... Sigma-Aldrich), air-dried, and stored at −80°C. Staining buffers were prepared and procedures performed as previously described ...

For the accumulation of pPyk2 Y402 and CD56 at the synapse of primary NK cells, masks of synaptic vs. non-synaptic actin were generated following auto thresholding of intensity in the actin channel.