and these target cells displayed rounding/detachment and cytoplasmic shrinkage, characteristic of progressing through apoptosis. Repeating the assay while using a fixed E:T of 4:1 clearly ...

Unexpectedly, we found that some of the assays widely used to detect apoptosis do not distinguish ... understand the mechanism of cell death. The Jurkat T-lymphoma and Ramos and Raji B-lymphoma ...

Apoptosis reagents must be diluted to ensure compatibility of apoptotic marker and target cell label, and treatments (such as cytokines, T cell stimuli, and antibodies) at 4 times final assay ...

The frequent use of assays to outline health and T cell function is vital for the advancement of these therapies and drugs. Sartorius has produced a high throughput, optimized flow cytometry assay ...

Studies carried out till date to elucidate the pathways involved in HIV-1-induced T-cell depletion has revealed that apoptosis underlie ... intracellular p24 antigen assay and HIV-1 gag-specific ...

But as most labs don't have EM technicians on staff ... Other than caspase activation, none of these tests indicates apoptosis so much as cell death in general. Reed's recommendations: run multiple ...

Characterization of antigen-specific T cell responses typically is a critical constituent of these immunological investigations which can also aid in patient stratification for future trials. As a ...

Cellular therapies could play a role in cancer treatment and regenerative medicine if it were possible to quickly eliminate the infused cells in case of adverse events. We devised an inducible T ...

Modulating lncRNA NKILA in tumor-reactive T cells to enhance their trafficking to breast cancer by inhibition of FasL-induced T-cell apoptosis. This is an ASCO Meeting Abstract from the 2017 ASCO-SITC ...

and efficient alternative to cell line target cells. This helps CAR-T developers accelerate assay validation and lot release, while avoiding the operational burden, variability and chain-of ...

Excitingly, these finding support that “this assay can be used in ongoing translational research studies to investigate CAR-T cell and TCR-T cell trafficking,” commented the authors. “Insight into ...

The risk of second tumors after chimeric antigen receptor (CAR) T-cell therapy ... and Shriya Syall for performing molecular assays.