Quantifying the release of UDP during biosynthesis reactions using a ‘UDP-Glo’ glycosyltransferase assay (Das et al., 2016) reveals Michaelis–Menten constants for CesA1 and CesA3 with respect to ...

Assessing variability in assay outputs provides foundational information to support the development of assays that provide optimal clinical performance for predicting treatment response. 16, 17, 23 A ...

Several assays have been developed to investigate the kinetics of GTs, but they suffer from drawbacks such as being end point assays or continuous assays with compromised specificity, sensitivity, or ...

The FDA has expanded the label for the VENTANA PD-L1 (SP263) Assay. The test is now an approved companion diagnostic to identify patients with non-small cell lung cancer (NSCLC) who are eligible for ...

Microbial assays are required for antibiotic assay in the US Pharmacopoeia and the British Pharmacopoeia. This official publication lists medicinal drugs, their effects, and directions for their use.

Keywords: glycosyltransferase, hepatocellular carcinoma, overall survival, prognostic signature, lasso regression analysis Citation: Zhou Z, Wang T, Du Y, Deng J, Gao G and Zhang J (2022) ...

The cell proliferation assay chosen for a study depends on its specific advantages and disadvantages for the reasons listed above (sensitivity, how well it can be reproduced, and compatibility ...

Post-translational modifications (PTMs), particularly glycosylation, affect a protein drug’s activity, effectiveness, and safety, but not always in predictable ways. Some glycans are known to be ...

Wound-Healing Assay A wound-healing assay was used to evaluate the migration of C33A and C33A-E6 cells. Briefly, cells (1 × 10 5) were seeded in 24-well plates for growth to 80–90% confluence. Then, ...

Rationale: In diabetic animals as well as high glucose cell culture conditions, eNOS (endothelial NO synthase) is heavily O-linked-N-acetylglucosaminylated (O-GlcNAcylated), which inhibits its ...