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Two-dimensional vibrational spectroscopy is applied to investigate the dilution process of 1-ethyl-3-methyl-imidazolium tetrafluoroborate ([Emim][BF4]) in water. With increasing water content in ionic ...

This is due to the lone pair of N7 being protected by hydrogen bonding in a protic solvent (G·H2O in our case), so the C8 position of guanosine will become more nucleophilic than position N7.