The field of cellular immunotherapy has undergone a profound transformation in recent years, with autologous chimeric antigen receptor (CAR) T-cell therapies emerging as the standard-of-care option ...

Step 3: HSC to NK cell differentiation Human CD34+ hematopoietic cells were seeded in pre-coated wells and cultured for 21 days in a medium containing TPO, Flt-3L, SCF, IL-7, and other factors ...

BACKGROUND: Evidence suggests that COVID-19 predisposes to cardiovascular diseases (CVDs). While monocytes/macrophages play a central role in the immunopathogenesis of atherosclerosis, less is known ...

Immunometabolic reprogramming due to adenosine produced by CD73 (encoded by the 5’-ectonucleotidase gene NT5E) is a recognized immunosuppressive mechanism contributing to immune evasion in solid ...

Flow Cytometry Isolated cells were stained with Live/Dead Fixable Dead Cell Stain (ThermoFisher Scientific) and marker staining performed with panels of surface or intracellular marker antibodies ...

In hematologic oncology, advancements in chimeric antigen receptors (CARs) for T-cell therapy have led to new investigations and an emerging role for CAR-natural killer (NK) cell therapy. Here we ...

Natural killer (NK) cells are cytolytic effector lymphocytes that participate in the surveillance of stressed, infected, or cancerous cells (Orange et al, 2006; Orange, 2008; Vivier et al, 2011). NK ...

Subsequently, KIR2DL1‐expressing cells were isolated by staining the entire NK cell population with anti‐KIR2DL1‐PE antibody (Miltenyi Biotec), followed by magnetic separation using the EasySep™ human ...

Immunofluorescence staining on human RCC samples demonstrated the advantages of NKp30 targeting versus CD56 for detection of tumor infiltrating NK cells. This work advances PET imaging of NK cells and ...

Human natural killer (NK) cells are defined as CD56 + CD3 −. Despite its ubiquitous expression on human NK cells the role of CD56 (NCAM) in human NK cell cytotoxic function has not been defined. In ...